Authors : Dr. Ujjwala Supe
Abstract – The present study deals with the In Vitro Callus induction of Bryonia Laciniosa whichis an important medicinal plant which belongs to the family Cucurbitaceae. It is widely used for inflammation, cough, flatulence and skin diseases. The efficient in vitro Callus induction of Bryonia Laciniosa was achieved from internode and leaf, explants on MS medium with different concentrations and combinations of 2,4-D and Kin. The highest rate of Callus induction was observed from the leaf explants on MS medium with 2,4-D (3 mg/l) + Kin (1 mg/l). The regenerated callus were transferred in to half strength MS medium fortified 2,4-D for Callus elongation. The elongated Callus was successfully the current study showed efficient in vitro Callus induction capabilities of Bryonia Laciniosa.
